STUDY OF THE OPTIMUM CONDITIONS FOR THE PRODUCTION OF BACTERIOCIN FROM LACTOBACILLUS BULGARICUS

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Kurdstan A. Abd Al-Karem
Suzan Kamran Hassan

Abstract

The optimum conditions for the production of bacteriocin from Lactobacillus bulgaricus in the liquid MRS medium were studied and NISIN was used as a standard bacteriocin. The concentration was measured on the principle of a spectrophotometer, where it was shown that the best carbon source is glucose at 3% and a better study Conditions from nitrogen sources in terms of ammonium citrate, which showed that the ratio of 0.3% was optimum for production, while for the nitrogen source peptone and meat extract the ratio was 1.5% and the nitrogen source for yeast extract was 0.7% was optimum, while the sources of salts were the optimum conditions for manganese sulfate and magnesium sulfate It was 0.01%, while acid dipotassium phosphate was 0.2%, with a inoculum size of 7×107.
Keywords: lactic acid, bacteria, Bacteriocin, lactobacillus bulgaricus.

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How to Cite

STUDY OF THE OPTIMUM CONDITIONS FOR THE PRODUCTION OF BACTERIOCIN FROM LACTOBACILLUS BULGARICUS. (2022). Iraqi Journal of Agricultural Research, 26(1), 210 to 220. https://doi.org/10.65814/ijar.2022.26.1.210

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