THE EFFECT OF BENZYL ADENINE AND NAPHTHALENE ACETIC ACID ON CALLUS GENERATION AND PERPETUATION IN Momordica Charantia IN VITRO

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Published: 2024-07-25
DOI: https://doi.org/10.65814/ijar.2024.28.1.22

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Nibras Mahdi Saleh

Abstract

This study was conducted in the Laboratory of the cell and plant tissue culture in the Department of Biology at the College of Education for pure Sciences, University of Diyala, during the period from 2020 until 2021 to study the effect of some growth regulators on the induction of callus of Momordica charantia. (leaves and cotyledons of Momordica charantia) (at three weeks of age) and different growth regulators: BA (0.0,0.5 and1.0) mg.l-1 on induced callus And the interaction between (BA) (1.0and2.0)mg.l-1 and (NAA) (0.5mg.l-1)at callus propagated stage. Also, BA at 0.5 and 1.0 mg.l-1 was tested at regeneration stage. The result showed the medium MS supplemented with a concentration of 0.5 mg.l-1 BA gave the highest response rate for callus induction from leaves at 60% and gave the highest fresh weight rate of 2.4g from cotyledons, after four weeks of culture. The interaction between 2.0 mg.l-1BA and 0.5mg.l-1 NAA gave the highest callus maintenance from recorded values of 2.74 and 5.72gm for both true and cotyledon leaves respectively. Callus of true leaves of bitter gourd showed the ability to Differentiation and formation of vegetative branches, gave the highest number of 4 shoots. explant-1 and the number of leaves 9.5 leaves part branches on MS medium supplemented with a concentration of 1.0 mg.l-1 BA.

Keyword: Callus Induction, Micropropagation, Momordica charantia

Article Details

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Vol. 28 No. 1 (2024): Iraqi Journal of Agricultural Research

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Articles

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Copyright (c) 2024 Nibras Mahdi Saleh (Author)

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How to Cite

THE EFFECT OF BENZYL ADENINE AND NAPHTHALENE ACETIC ACID ON CALLUS GENERATION AND PERPETUATION IN Momordica Charantia IN VITRO . (2024). Iraqi Journal of Agricultural Research, 28(1), 22-30. https://doi.org/10.65814/ijar.2024.28.1.22

References