EVALUATE SOME OF CHEMICAL AND BIOLOGICAL AGENTS IN CONTROLLING ROOT ROT DISEASE OF COWPEA Vigna unguiculata (L.)Walp
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Abstract
This research was aimed to identify the causal agent of root rot disease of cowpea in three Iraqi provinces of Qadisia, Babil and Diyala, and evaluate its pathogenicity and control by using beneficial bacteria of Bacillus cereus (Bc) and Pantoea agglomerans (Pa) which isolated from the rhizosphere of healthy cowpea plants, and silicon (Si) and copper (Cu). The fungus Fusarium solani was predominant, while its percentage of appearance was 82.2% with frequency of 52.7%, Isolate Bfs-7 was most virulent which prohibited germination of the cowpea seed totally in vitro compared to control. The bacterial isolates D6 and Q10 exhibited 100% percentage of inhibition against the pathogen in vitro. Results of bacterial isolates identification using Vitiq2 Compact System showed that they belong to B. cereus and P. agglomerans respectively. Under greenhouse conditions the treatment with (Bc+Pa+Si+Cu) was superior in controlling the disease, while it exhibited 100% percentage of seed germination compared to the negative control (Fungus alone) which achieved 52.2% and the percentage of disease incidence and severity were 7.5%, 1.0% respectively compared to the negative control which were 95.0%, 70.5% respectively. And it exhibited significant increase of plant growth criteria represented by the dry width of plant, while it achieved 2.03 gm/plant compared to negative and positive control which were 0.88, 1.76 gm/plant respectively.
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