TOXICOLOGICAL AND BIOCHEMICAL EFFECTS OF IMIDACLOPRID

Abstract

Application of pesticides on vegetables will protect them from pest injury, but in another hand will hold pesticide residues in vegetables. These residues have harmful effect against all consumers. In the present study, we took imidacloprid insecticide, because it used widely for combatant vegetable insect control in Iraq. Median lethal dose (LD50) for imidacloprid pesticide was determinant by using Probit analytical method. The evaluation of toxicity carried out by taking 1/10, 1/100, and 1/10000 from the LD50 of imidacloprid insecticide. Orally administration of determinant doses applied on albino mice using gavage-tube for 30 days as sub-chronic exposure. We took both biochemical and hematological parameters, as indicators for insecticide toxicity. The histopathological study been carried out for liver, kidney, and spleen after we sacrifice the mice of each group separately in the end of the sub- chronic orally administration period. ANOVA statistical test carried out for results of imidacloprid biochemical and hematological results. The results varied based on kind of parameters and concentrations, low concentrations dose 1/10, 1/100 and 1/10000 from LD50 has the same or even more adversely effect towards targeted organs, the reason for that attitude due to bio- accumulation effect and the inability of low dose for stimulate the defense systems of body like superoxide dismutase(SOD), whereas, intermediate and high dose concentration able to stimulate body defense systems like SOD.