عزل وغربلة وتشخيص العزلات المحلية لبكتريا Bacillus sp المنتجة لأنزيم اللاكيز (Laccase)

الملخص

Fifty bacteria isolates that produce Laccase enzyme were obtained from
200 isolate and seems to belongs to (Bacillus sp.) were isolated from different
local sources (soil samples from Al-Khadra, Al-Iskan and Abu Ghraib areas) in
baghdad; the obtained isolates were screened for their capabilities for producing
Laccase enzyme . It has been noticed that isolates designated (38, 41, 45, 49, 60,
104, 123, 133, 136 and 143) were showed high capability for producing Laccase
enzyme at 37° C in terms of the time consumed to interact with the SGZ reagent.
All isolates subjected to secondary screening. The results revealed that isolate
(136) was the highest producers with enzyme activity of (47.0 U/ml). The
Identification tests of this isolate were carried out by studying morphological,
microscopic characteristic and using of Vitek2 compact system. These tests were
conformed by identification of 16S rRNA gene using PCR technique and its
nitrogen base sequencing and the results revealed that the isolate was belongs to
Bacillus subtilis bacteria.