COLD EXTRACTION OF CITRUS-PEELS OIL AND DETERMATION OF ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES
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Abstract
exposure to chemical , physical and biological tests to achieve this goal , the
percentage of oil extraction was 30% and that detecting process of phenols
showed that oil contain phenols and the result was positive with ferric chloride
detector by appearance the blue color and the quantity of whole phenols in oil
was 40 (GAE. mg /g), and the PV for oil in concentration 50 ppm and 100 ppm
was 5.8 and 5.5 respectively , when evaluation phenolic compounds in citrus
peels oil by using HPLC and GC, the phenolic compounds was limonene , alpha
penen and beta-penen with concentration 91.7%, 2.89% and 5.24% respectively ,
when evaluation antimicrobial activity for oil in concentration (0.5, 1, 2 and 3)%,
the diameter of clear zone was 2,4,7 and 11 (mm) respectively against salmonella
typhi bacteria and 5,10,14 and 13 (mm) respectively against staphylococcus
aureus bacteria and 5,11,13 and 11 (mm) respectively against E.coli bacteria and
4 ,11 , 1 and 11 (mm) respectively against Bacillus subtillus bacteria and in same
concentration the diameter of clear zone against Aspergillus niger mold was 4, 9,
15 and 12 (mm) respectively and 5,9,15 and 12 (mm) against candida albicans
yeast, all them with differences on p(0.05), the minimum inhibition
concentration to extracted
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Copyright (c) 2018 Hanin Abdul Amir Al-Asadi, Ramadan Najm Al-Saadi (Author)
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