THE EFFECT OF BA AND KIN GROWTH REGULATORS ON CALLUS INDUCTION IN Rosmarinus officinalis L. in vitro
Article Sidebar
Main Article Content
Abstract
A tissue culture technique was used to micropropagation the rosemary plant Rosmarinus officinalis L. by initiating callus generation, formation and maintenance from single node parts. The experiments included the use of two types of sterilization, mercuric chloride (HgCl2) at a concentration of (0.1 and 0.06%) and a sterilization period of (15 and 10) minutes and sodium hypochlorite (NaOCl) at a concentration of (10%) and a sterilization period of (10 and 15) minutes and planted in MS nutrient medium. The effect of adding BA and Kin at a concentrations of (0.0, 0.5, 1.0, 2.0) mg.L-1 on callus initiation was also studied. The effect of the interaction between BA at a concentrations (1.0) mg.L-1 and Kin at concentration (2.0) mg.L-1 + (1.5, 2.0) mg.L-1 2,4-D was tested in replanting and maintaining callus. The results showed that the type of material used in sterilization has a significant effect in reducing the percentage of contamination of ex plant grown in vitro. The use of sodium hypochlorite (NaOCl) at a concentration of (10%) v\v for 15 minutes, was effective in reducing the percentage of contamination to the lowest level, as the percentage of survival in the planted and sterilized plant part reached this material 85%. The results showed that the highest percentage and fresh weight rate for callus induction value reached 80% and 4.56g in the medium supported with a concentration of (1.0) mg.L-1 BA, while the response rate and fresh weight rate achieved a value of 60% and 3.82g in the medium supplied with a concentration of 2.0 mg.L-1 Kin while the comparison treatment did not record any fresh weight rate for the induction callus. As for the effect of the interaction of the nutritional medium provided with a concentration of (1.0) mg.L-1 BA+ (2.0) mg.L-1 2,4-D the highest fresh weight was recorded reaching7.35g which was significantly superior to the interaction between the treatment between (2.0) mg.L-1 Kin+ (2.0) mg.L-1 2,4-D with an average fresh weight of 5.37g respectively.
Article Details
Section
License
Copyright (c) 2025 Nbras Saleh Mahdi Almahdi (Author)
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright and Licensing Policy
Iraqi Journal of Agricultural Research (IJAR) adheres to the highest standards of transparency and integrity in academic publishing. The IJAR follows an Open Access policy to ensure the global exchange of knowledge:
- Ownership of Copyright:
Ø Authors retain full copyright of their work published in the IJAR without any restrictions.
Ø Authors grant the IJAR the "right of first publication" for their work.
- Licensing:
Ø All articles are published under the Creative Commons Attribution- 4.0 International License (CC BY 4.0).
Ø This license allows others to share, adapt, and build upon the work provided the original author and the IJAR are properly credited.
- Self-Archiving Policy:
Ø Authors are encouraged to deposit the version of record (Publisher's PDF) in their institutional repositories or personal websites immediately upon publication, provided that a link to the original DOI is included.
- Originality:
Ø By submitting a manuscript to the IJAR, authors certify that the work is original, has not been published previously, and is not under consideration by any other journal.
Ø The IJAR does not allow the violations of others’ intellectual property rights, and authors must comply with the requirement to cite all references.